3th Imaging Academy (Day 2)

Molecular and cell biologist from training, Laurianne has worked with High Content Screening equipment during her postdocs. She joined PerkinElmer four years ago and continues since supporting scientists developing HCS assays around the world.

From more than 10 years focused on HCS systems configuration according to users needs

As a highly experienced researcher in cell biology and biochemistry, he has spent over a decade designing and executing both commercial and academic research projects. He possesses a wide range of expertise in cellular models and state-of-the-art in vitro methods with a particular focus on High Content Screening approaches and single cell analysis.

Natalia Karczewska is a biologist at the Centre for Chemical Biology ERIC in the Institute of Bioorganic Chemistry of the Polish Academy of Sciences in Poznan. The Centre has experience and provides services in HTS of compound libraries, multi-parametric high-content imaging, super-resolution microscopy, as well as chemical synthesis of bioactive molecules and “Hit to Lead” approach to molecular optimization.

She graduated in 2022 with a MSc in Biotechnology from Adam Mickiewicz University in Poznan. At the Centre, she works on projects related to the topic of multiparametric microscopic imaging, including the use of “Cell Painting” assay on adherent human cell lines. In addition, she focuses on developing skills in the analysis of microscopic images, as well as the analysis of multiparametric numerical data from compound screening performed using microscopic imaging.

Małgorzata Wieteska: With an MSc in Biology from the University of Warsaw, is currently pursuing her doctoral studies at the Doctoral School of Information and Biomedical Technologies (TIB). With a decade of experience as a Lab Technician and Researcher at the Nencki Institute of Experimental Biology, Polish Academy of Sciences, she has contributed to various research projects. In her current role as a Researcher for the National Science Center (NCN) project, she oversees tasks such as monitoring sample conditions during cytotoxicity tests and conducting analyses using a high-throughput microscopy. Her expertise in cell biology and biochemistry reflects a dedicated commitment to advancing scientific inquiry through innovative in vitro methods.

Monika Pyc is a biologist working at the Centre for Chemical Biology ERIC, part of the Institute of Bioorganic Chemistry at the Polish Academy of Sciences in Poznań. The Centre specializes in a range of services, including high-throughput screening (HTS) of compound libraries, multi-parametric high-content imaging, super-resolution microscopy, as well as the chemical synthesis of bioactive molecules and the "Hit to Lead" strategy for molecular optimization.
She earned her MSc in Medical Biotechnology from the Medical University of Silesia in 2018. At the Centre, her work focuses on projects related to multiparametric microscopic imaging. This includes applying the "Cell Painting" assay to adherent human cell lines, optimizing cell-based assays, adapting processes to HTS formats, and automating workflows for high-throughput screening in biochemical models.

After obtaining MSc degree in biotechnology from Warsaw University of Life Sciences (SGGW), I began a PhD project at the Spanish National Cancer Research Centre (CNIO) in Madrid, where I investigated molecular mechanisms underlying the development of pancreatitis and pancreatic adenocarcinoma. Subsequently, I moved to International Institute of Molecular and Cell Biology (IIMCB) in Warsaw, where I studied the crosstalk between intracellular trafficking, cell metabolism and differentiation. Currently I am employed at the Maria Sklodowska-Curie National Research Institute of Oncology in Warsaw (MSCI), where I am developing HCS-based assays to study cancer cell metabolism and drug sensitivity.

Grzegorz jest absolwentem Wrocławskiego Uniwersytetu Medycznego, gdzie studiował na Wydziale Farmaceutycznym. W 2007 roku uzyskał stopień doktora nauk biologicznych w Instytucie Immunologii i Terapii Doświadczalnej PAN we Wrocławiu na podstawie badań nad właściwościami adiuwantowymi laktoferyny w preparatach szczepionkowych. W latach 2005-2007 pracował jako pracownik naukowy w University of Texas Medical Branch w Galveston w USA w laboratorium dr. Istvana Boldogha, badając rolę reaktywnych form tlenu w reakcjach alergicznych. W 2007 roku rozpoczął staż podoktorski na University of Texas MD Anderson Cancer Center w Houston w USA, w laboratorium dr. Tomasza Zala. Jego projekt obejmował mikroskopową wizualizację aktywności układu immunologicznego skóry i mikrośrodowiska nowotworu płuc. W 2011 roku objął łączone stanowisko kierownika usługowej pracowni mikroskopowej w La Jolla Institute for Immunology w San Diego, USA oraz instruktora w laboratorium dr. Mathiasa von Heratha, gdzie prowadził badania obrazowe nad udziałem układu odpornościowego w przebiegu wielu chorób (cukrzyca typu 1, infekcje wirusowe, choroba Leśniowskiego-Crohna, miażdżyca i nowotwory). W 2014 roku dołączył do Wrocławskiego Centrum Badań EIT+ (obecnie Łukasiewicz-PORT), aby pomóc w tworzeniu laboratorium zajmującego się bioobrazowaniem. W 2023 roku założył własną grupę badawczą zajmującą się immunoterapią nowotworów. Jego celem jest opracowanie innowacyjnych technologii, które mogą zwiększyć skuteczność immunoterapii.

Klaudia Leśniak has been employed at Selvita SA since 2018, currently holding the position of Senior Scientist I in the Cell and Molecular Biology Department. With over four years of experience, she has played a key role in projects involving High Content Screening. She is responsible for the development and high-throughput screening of various assays, including:
• Live and fixed cell staining
• Proliferation assays and cell cycle analysis
• Senolysis assays on senescent cancer and primary cells
• Phenotype classification using PhenoLOGIC machine learning
• Fluorescence in situ hybridization (FISH) studies on telomeric stability and DNA damage marker co-localization
• Automated Preci-scan of tissue samples
Klaudia holds a degree in Biochemistry from the Jagiellonian University in Kraków, Poland. Following her graduation, she completed internships in the "Functional Proteome Analysis" research group at the German Cancer Research Center (DKFZ), as well as at the Institute of Tumor Pathology at the University Clinic of Heidelberg.

Mikołaj is a PhD student at the Doctoral School of Biomedical
Sciences at the University of Wrocław, where his research
focuses on understanding how the adipogenic niche
(adipocytes and adipose stromal cells) influences cancer
progression. He investigates how various 2D and 3D in vitro
models can provide deeper insights into the interactions
between tumors and their microenvironment. In his work, he
uses live-cell imaging techniques to analyze the behavior and
interactions of cancer cells, aiming to offer a more
comprehensive understanding of tumor biology.